NAD+ (500mg/10mL Vial) Dosage Protocol

NAD+ (500 mg Vial) Dosage Protocol

Quickstart Highlights

NAD+ 500 mg dosage protocol supports cellular energy production and healthy metabolic function through subcutaneous (Sub-Q) or intramuscular (IM) injections.

  • Typical starting dose 10 mg (0.1 mL) per injection, 2-3× weekly
  • Gradual titration to 25 mg → 50 mg per injection as tolerated
  • Reconstitute with 5 mL bacteriostatic water (100 mg/mL concentration)
  • Injection volume remains practical: 10–50 units on a U-100 insulin syringe
  • Store lyophilized at −20 °C; refrigerate reconstituted solution at 2–8 °C
NAD+ 500 mg Vial

Dosing & Reconstitution Guide

Educational guide for reconstitution and titration protocol

Standard / Gradual Approach (5 mL = 100 mg/mL)

Phase (Weeks) Dose per Injection (mg) Units (≈ mL)
1 – 2 10 mg 10 u (0.10 mL)
3 – 4 25 mg 25 u (0.25 mL)
5 + 50 mg 50 u (0.50 mL)

Inject 2 – 3 times weekly (e.g., Mon/Wed/Fri). 5 mL reconstitution keeps syringe markings easy to read; increase to 10 mL (50 mg/mL) if finer measurement is preferred.

  1. Draw 5.0 mL bacteriostatic water into a sterile syringe.
  2. Inject slowly down the vial wall to minimize foaming.
  3. Gently swirl until dissolved; avoid vigorous shaking.
  4. Label with date and store at 2–8 °C.
  5. Rotate injection sites and use new sterile syringes each time.

Research use only. This educational guide is not medical advice.

Protocol Overview

Concise outline of the 2-3×-weekly regimen.

  • Goal: Support cellular energy production and metabolic health
  • Schedule: 2-3 subcutaneous or IM injections per week
  • Dose Range: 10 mg → 50 mg per injection
  • Reconstitution: 5 mL (100 mg/mL) for practical syringe units
  • Storage: Freeze lyophilized; refrigerate after mixing

Dosing Protocol

Gradual titration improves tolerance.

  • Initial: 10 mg (0.10 mL) per injection, 2-3× weekly
  • Intermediate: 25 mg (0.25 mL) per injection
  • Target: 50 mg (0.50 mL) per injection
  • Cycle Length: Continue indefinitely for research objectives
  • Timing: Any time of day; maintain consistent schedule

Storage Instructions

Correct storage maintains compound integrity.

  • Lyophilized: −20 °C (freezer) until reconstitution
  • Reconstituted: 2–8 °C (refrigerator) up to 30 days
  • Protect from light and avoid repeated freeze-thaw cycles

Supplies Needed

Approximate quantities for an 8- to 16-week research cycle.

  • Peptide Vials:
    • 8 wks ≈ 2 vials
    • 12 wks ≈ 3 vials
    • 16 wks ≈ 4 vials
    (Adjust if using lower or higher dosing frequencies)
  • Insulin Syringes (U-100):
    • 8 wks ≈ 24
    • 12 wks ≈ 36
    • 16 wks ≈ 48
  • Bacteriostatic Water: 1 × 30 mL bottle
  • Alcohol Swabs: 1 box
NAD+ Vial
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Insulin Syringes
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Bacteriostatic Water
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Alcohol Pads
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Important Notes

Practical tips for accurate and consistent research.

  • Rotate injection sites and use new sterile syringes each time.
  • Maintain steady scheduling for reproducible results.
  • Monitor for irritation; adjust volume or syringe size if necessary.
  • Document all observations for rigorous study records.

How This Works

NAD+ is a redox coenzyme essential for mitochondrial ATP production and cellular repair pathways.

  • Energy Metabolism: Accepts and donates electrons within the electron transport chain
  • Sirtuin Activation: Serves as a substrate for sirtuin enzymes involved in DNA repair
  • Cellular Signaling: Modulates PARP activity for genomic stability

Potential Benefits & Side Effects

Reported observations vary; below are common research findings.

  • Enhanced cellular energy and reduced fatigue
  • Improved cognitive clarity and focus
  • Support for healthy aging and DNA repair processes
  • Possible mild injection-site irritation; systemic side effects are rare

Lifestyle Factors

Complementary practices to optimize outcomes.

  • Follow a nutrient-dense diet and stay hydrated
  • Engage in regular physical activity with adequate recovery
  • Prioritize sleep and stress-management techniques

Injection Technique

Simple guidelines for safe daily injections.

  • Clean vial rubber stopper & injection site with alcohol swabs
  • Insert needle at a 45–90° angle into subcutaneous tissue
  • Inject slowly & rotate sites (abdomen, thigh, etc.)

Important Note

This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.

References


  • University of Maryland Archive
    – NAD+ Mechanism and Clinical Insights
    View Source

  • PMC
    – Study on NAD+ and Aging
    View Source

  • PMC
    – NAD+ Role in Cellular Function
    View Source

  • medRxiv Preprint
    – Preliminary Results on NAD+ Trials
    View Source

  • PMC
    – Effects of NAD+ on Metabolism
    View Source