MOTS-C (5mg Vial) Dosage Protocol

Quickstart Highlights

MOTS-C 5mg dosage protocol is a mitochondrial-derived peptide that may support metabolic regulation, exercise performance, and healthy aging through periodic subcutaneous injections.

  • Common research use: 5 mg every 5 days or 10 mg per injection (advanced)
  • Flexible dosing schedule (e.g., once every 5 days or 3x weekly)
  • Reconstitute for accurate insulin syringe measurements (up to 3 mL max)
  • Store lyophilized in the freezer; reconstituted in the refrigerator
MOTS-C Vial

Dosing & Reconstitution Guide

Educational guide for reconstitution and dosing protocol

Standard / Gradual Approach (1 mL = 5 mg/mL)

Injection # Single Dose (mg) Units (per injection)
Injection 1 (Day 1) 5 mg 100 units (1.0 mL)
Injection 2 (Day 6) 5 mg 100 units (1.0 mL)
Injection 3 (Day 11) 5 mg 100 units (1.0 mL)
Injection 4 (Day 16) 5 mg 100 units (1.0 mL)

A common research protocol involves 5 mg injections every 5 days (4 total injections over ~20 days). Reconstitute each 5mg vial with 1 mL of bacteriostatic water to achieve a 5 mg/mL solution. Each injection uses the entire vial (1.0 mL).

  1. Draw 1.0 mL of bacteriostatic water into a sterile syringe.
  2. Inject the water slowly along the vial wall to minimize foam.
  3. Gently swirl—avoid vigorous shaking.
  4. Label the vial with the date and store at 2–8°C (refrigerator).
  5. Use within 30 days; avoid repeated freeze-thaw cycles.

Advanced / Aggressive Approach (Requires 2 Vials per Injection)

Week Injections/Week Single Dose (mg) Combined Volume
Weeks 1–4 3x weekly (e.g., M/W/F) 10 mg ~1.0 mL total

For higher total weekly dosing (10 mg per injection), each injection combines 2 vials (5 mg each).
Reconstitution Example: Reconstitute each 5mg vial with 0.5 mL of bacteriostatic water so each vial is 5 mg in 0.5 mL. Draw up both vials (0.5 mL + 0.5 mL) into one syringe (1.0 mL total) = 10 mg.
If using standard 1mL insulin syringes (100 units max), the injection volume will be 1.0 mL (100 units).

  1. Draw 0.5 mL of bacteriostatic water into a sterile syringe and inject into the first 5mg vial.
  2. Repeat with a second vial to reconstitute it separately.
  3. Slowly combine both 0.5 mL solutions into a single syringe for a total of 1.0 mL (10 mg).
  4. Refrigerate each reconstituted vial at 2–8°C and use within 30 days.
  5. Avoid multiple freeze-thaw cycles.

Note: This approach may require multiple vials over the entire cycle.

Note: This guide is for educational purposes only. Always consult with a qualified healthcare professional.

Protocol Overview

A concise summary of the MOTS-C protocol for metabolic and performance research.

  • Goal: Investigate potential metabolic regulation and exercise performance benefits
  • Schedule: Subcutaneous injections every 5 days or 3x per week, depending on protocol
  • Dose Range: 5 mg to 10 mg per injection
  • Reconstitution: 1–2 vials may be combined for advanced dosing
  • Storage: Keep lyophilized vials frozen; refrigerate after reconstitution

Dosing Protocol

Suggested approaches include a moderate “standard” schedule or an “advanced” higher dose regimen.

  • Standard Frequency: Inject 5 mg once every 5 days (4 total injections over ~20 days)
  • Advanced Frequency: Inject 10 mg up to 3 times weekly (2 vials per injection)
  • Cycle Length: Varies by research design (e.g., 4–8 weeks or longer)
  • Timing: Subcutaneous administration; consistent injection timing suggested

Storage Instructions

Proper storage ensures peptide integrity.

  • Lyophilized: Freeze until mixing (−20°C)
  • Reconstituted: Refrigerate at 2–8°C
  • Use within 30 days of reconstitution
  • Avoid repeated freeze-thaw cycles

Supplies Needed

Adjust quantities based on injection frequency and cycle length.

  • Peptide Vials (5mg each):
    • Standard (4 injections) = ~4 vials total
    • Advanced (3x weekly for 4 weeks) = ~24 vials total (2 per injection, 12 injections)
  • Insulin Syringes:
    • One syringe per injection
  • Bacteriostatic Water: 1× 30ml
  • Alcohol Swabs: 1 box
MOTS-C Vial View Supplier
Insulin Syringes View Supplier
Bacteriostatic Water View Supplier
Alcohol Pads View Supplier

Important Notes

Practical tips to enhance safety and efficacy.

  • Always use new, sterile insulin syringes & rotate injection sites.
  • Maintain consistency; follow the planned schedule for reliable results.
  • Observe for adverse reactions; consult a professional if concerns arise.
  • Track progress throughout the protocol, adjusting dosage as recommended.

How This Works

MOTS-C is a mitochondrial-derived peptide that may help regulate energy balance, glucose metabolism, and exercise performance.

  • Metabolic Support: Influences insulin sensitivity and cellular energy pathways
  • Physical Performance: May improve exercise capacity via mitochondrial function
  • Possible anti-inflammatory effects noted in some research contexts

Potential Benefits & Side Effects

Most research indicates improvements in metabolism and endurance, though individual responses vary.

  • May support healthier glucose regulation and energy expenditure
  • Potential to enhance physical performance and recovery
  • Possible mild side effects include fatigue, headache, or local injection-site irritation
  • Rarely, oxidative stress or tissue fatigue could occur with overuse

Lifestyle Factors

Complementary strategies for maximum benefit.

  • Adopt a balanced, nutrient-dense diet
  • Engage in consistent exercise and prioritize adequate rest
  • Manage stress levels and maintain good hydration

Injection Technique

Simple guidelines for safe daily injections.

  • Clean vial rubber stopper & injection site with alcohol swabs
  • Insert needle at a 45–90° angle into subcutaneous tissue
  • Inject slowly & rotate sites (abdomen, thigh, etc.)

Important Note

This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.

 

References

  • PubMed Central – Mitochondrial peptide regulation study
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  • EMBO Molecular Medicine – MOTS-c and metabolic function
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  • Nature Communications – Mitochondrial peptide benefits
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  • PubMed Central – MOTS-c metabolic improvement research
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  • PubMed Central – Studies on mitochondrial-derived peptides
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  • PubMed Central – Recent findings on MOTS-c
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  • Alzheimer’s Drug Discovery – Cognitive Vitality review of MOTS-c
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  • Frontiers in Physiology – MOTS-c research article
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  • bioRxiv – Preprint on MOTS-c function
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  • Diabetes & Metabolism Journal – Examination of MOTS-c in diabetes
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  • PubMed Central – Investigations on MOTS-c efficacy
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  • Frontiers in Endocrinology – Endocrine impacts of MOTS-c
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  • Aging (Albany NY) – MOTS-c and cellular aging
    View Source
  • USADA – Anti-doping overview of MOTS-c
    View Source
  • Physiological Reports – Peer-reviewed MOTS-c analysis
    View Source
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