Glutathione (600mg Vial) Dosage Protocol
Quickstart Highlights
Glutathione 600mg dosage protocol supports systemic antioxidant defenses, healthy skin, and recovery from oxidative stress through precise Sub-Q or IM injections.
- Typical dose range: 100 mg – 200 mg per injection
- Common frequency: 2–3 injections per week
- Standard reconstitution: 3 mL (200 mg/mL) for easy 25-50 unit draws
- High-dose clinical protocols up to 600 mg (split injections) exist
- Store lyophilized vials frozen; refrigerate after reconstitution

Dosing & Reconstitution Guide
Educational guide for reconstitution and dosing protocol
Standard / Gradual Approach (3 mL = 200 mg/mL)
Phase | Weekly Dose (mg) | Approx. Units (per injection) |
---|---|---|
Weeks 1–4 | 200 mg (100 mg × 2) | 50 u (0.5 mL) |
Weeks 5–8 | 400 mg (200 mg × 2) | 100 u (1.0 mL) |
Weeks 9–12 (Optional) | 400 mg – 600 mg | 100 u – 150 u |
Ideal for sustained antioxidant support. Inject 2 times per week (e.g., Mon & Thu). Reconstitute each 600 mg vial with 3 mL bacteriostatic water to create a 200 mg/mL solution—yielding 25–150 insulin-units per shot depending on dose.
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly along the vial wall; swirl gently until fully dissolved.
- Label vial with date & concentration (200 mg/mL).
- Refrigerate at 2–8 °C.
Advanced / Aggressive Approach (2 mL = 300 mg/mL)
Phase | Weekly Dose (mg) | Approx. Units (per injection) |
---|---|---|
Weeks 1–4 | 600 mg (200 mg × 3) | 67 u (0.67 mL) |
Weeks 5–8 | 600 mg (300 mg × 2) | 100 u (1.0 mL) |
Reserved for clinically supervised applications (e.g., chemotherapy support, male infertility). Reconstitute with 2 mL to reduce volume. If a single draw exceeds 60 units, split into separate injections and rotate sites.
- Draw 2.0 mL bacteriostatic water.
- Slowly add to vial; swirl gently—avoid foaming.
- Document 300 mg/mL on the label; refrigerate.
Protocol Overview
Concise summary of the standard twice-weekly antioxidant regimen.
- Goal: Boost systemic antioxidant capacity & support recovery
- Schedule: Sub-Q or IM injections 2× weekly
- Dose Range: 100 mg – 200 mg per injection (200 – 400 mg weekly)
- Reconstitution: 3 mL (200 mg/mL) for convenient 25–100 unit draws
- Storage: Freeze lyophilized; refrigerate reconstituted vial
Dosing Protocol
Suggested moderate plan for wellness & skin support.
- Per-Injection Dose: Start 100 mg (50 u); titrate to 200 mg as needed
- Frequency: Every 3–4 days (e.g., Mon/Thu)
- Cycle Length: 8–12 weeks, followed by 2–4 weeks break
- Maximum Weekly: 600 mg (split injections)
- Timing: Any time of day; inject slowly for comfort
Storage Instructions
Proper storage ensures peptide integrity.
- Lyophilized: Store at −20 °C (freezer) until use
- Reconstituted: 2–8 °C (refrigerator) up to 30 days
- Protect from light; avoid repeated freeze-thaw
- Discard solution if cloudy, discolored, or past expiry
Supplies Needed
Approximate quantities for antioxidant protocol.
- Peptide Vials:
• 8 wks ≈ 3 vials
• 12 wks ≈ 5 vials
• 16 wks ≈ 7 vials - Insulin Syringes: Match number of injections (e.g., 2× weekly × 12 wks = 24 syringes)
- Bacteriostatic Water: 1 × 30 mL bottle
- Alcohol Swabs: 1 box
Important Notes
Practical tips to enhance safety and accuracy.
- Rotate injection sites; allow solution to reach room temperature to reduce sting.
- If <10 units per dose is unavoidable, consider 50- or 30-unit syringes for precision.
- Split injections if draw volume exceeds 0.6 mL (≈60 units).
- Monitor zinc status during prolonged high-dose use.
- For research use only—not approved for human therapy without a licensed professional.
How This Works
Glutathione is a tripeptide (γ-l-glutamyl-l-cysteinyl-glycine) that serves as the body’s master antioxidant.
- Redox Balance: Neutralizes reactive oxygen species and recycles other antioxidants (e.g., vitamin C, vitamin E).
- Detoxification: Conjugates xenobiotics in the liver via glutathione-S-transferase pathways.
- Cellular Repair: Supports DNA synthesis and immune function, aiding recovery from oxidative damage.
Potential Benefits & Side Effects
Users often report improved skin tone, energy, and faster recovery—while adverse reactions are typically mild.
- Supports healthy immune response & detox pathways
- May improve skin brightness & collagen quality
- Possible mild side effects: injection-site redness, transient sulfur taste, nausea
- Rare: dizziness, allergic rash—monitor and consult a professional if concerns arise
Lifestyle Factors
Complementary strategies enhance results.
- Consume micronutrient-rich foods (selenium, vitamins C & B6 support endogenous GSH).
- Maintain regular exercise and adequate recovery to modulate oxidative stress.
- Prioritize sleep and stress management to preserve natural antioxidant reserves.
Injection Technique
Simple guidelines for safe daily injections.
- Clean vial rubber stopper & injection site with alcohol swabs
- Insert needle at a 45–90° angle into subcutaneous tissue
- Inject slowly & rotate sites (abdomen, thigh, etc.)
Recommended Source
We recommend Pure Lab Peptides for high-purity BPC-157 + TB-500 blend.
Why Pure Lab Peptides?
- Verifies ≥99% purity through independent lab testing
- Trusted by researchers seeking reliable results
- Follows rigorous manufacturing standards for consistent quality
Important Note
This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.
References
-
Hum Reprod
– GSH cross-over trial in male infertility -
Movement Disorders
– IV glutathione pilot study in Parkinson’s -
J Int Soc Sports Nutr
– GSH reduces exercise-induced muscle fatigue -
Clin Cosmet Investig Dermatol
– GSH antiaging and antimelanogenic effects -
Iran J Reprod Med
– Review of oxidative stress in male infertility