GHRP-6 (2 mg Vial) Dosage Protocol
Quickstart Highlights
GHRP-6 (Growth Hormone-Releasing Peptide 6) is a synthetic hexapeptide that functions as a potent growth hormone secretagogue by binding to the ghrelin receptor (GHS-R1a)[1][2]. It stimulates pulsatile GH release from the pituitary gland while maintaining physiological feedback controls, resulting in elevated IGF-1 levels and potential anabolic benefits[3]. This educational protocol presents a three-times-daily subcutaneous approach using practical dilution for precise insulin-syringe measurements.
- Reconstitute: Add 3.0 mL bacteriostatic water → ~0.67 mg/mL concentration.
- Typical daily range: 300–900 mcg total (split into 3 doses with gradual titration).
- Easy measuring: At 0.67 mg/mL, 1 unit = 0.01 mL ≈ 6.67 mcg on a U-100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 7 days.
Dosing & Reconstitution Guide
Educational guide for reconstitution and multiple-daily dosing
Standard / Gradual Approach (3 mL = ~0.67 mg/mL)
| Week/Phase | Dose per Injection (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 100 mcg | 15 units (0.15 mL) |
| Weeks 3–4 | 200 mcg | 30 units (0.30 mL) |
| Weeks 5–12 | 300 mcg | 45 units (0.45 mL) |
Frequency: Inject three times daily subcutaneously, spaced at least 4 hours apart (morning, midday, bedtime). GHRP-6 has a short half-life of approximately 2.5 hours[4], making multiple daily injections more effective than once-daily dosing for sustained GH elevation[5]. Each injection should be administered on an empty stomach (2–3 hours after meals, 30 minutes before eating) to maximize GH release[6].
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall to avoid foaming; do not shake.
- Gently swirl or roll the vial until the powder is completely dissolved.
- Label with date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
- Use within 7 days of reconstitution for optimal potency.
Supplies Needed
Plan based on a 12-week protocol with three-times-daily injections and gradual titration.
-
Peptide Vials (GHRP-6, 2 mg each):
- 12 weeks ≈ 32 vials (based on gradual titration to 300 mcg × 3 daily)
-
Insulin Syringes (U-100):
- Per week: 21 syringes (3/day × 7 days)
- 12 weeks: 252 syringes (recommend 3 × 100-count boxes)
-
Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.
- 12 weeks (32 vials): 96 mL → 10 × 10 mL bottles
-
Alcohol Swabs: One for the vial stopper + one for the injection site per injection.
- Per week: 42 swabs (2 per injection × 3 daily × 7 days)
- 12 weeks: 504 swabs → recommend 6 × 100-count boxes
Protocol Overview
Concise summary of the three-times-daily regimen.
- Goal: Stimulate pulsatile GH release to support muscle growth, fat loss, and recovery[2][7].
- Schedule: Three subcutaneous injections daily for 8–12 weeks, spaced at least 4 hours apart.
- Dose Range: 100–300 mcg per injection with gradual titration (300–900 mcg total daily).
- Reconstitution: 3.0 mL per 2 mg vial (~0.67 mg/mL) for accurate unit measurements.
- Storage: Lyophilized frozen; reconstituted refrigerated; use within 7 days.
Dosing Protocol
Suggested titration approach with multiple daily doses.
- Start: 100 mcg per injection, 3× daily (300 mcg total) for Weeks 1–2.
- Increase: 200 mcg per injection, 3× daily (600 mcg total) for Weeks 3–4.
- Target: 300 mcg per injection, 3× daily (900 mcg total) for Weeks 5–12.
- Timing: On empty stomach; space injections at least 4 hours apart; wait 30 minutes before eating.
- Cycle Length: 8–12 weeks; rotate injection sites to prevent tissue irritation.
Storage Instructions
Proper storage preserves peptide quality and potency.
- Lyophilized: Store at −20 °C (−4 °F) in dry, dark conditions with desiccant if available.
- Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within 7 days[8].
- Avoid freeze-thaw cycles: Do not refreeze reconstituted solution; prepare aliquots if extended storage is needed.
- Allow vials to reach room temperature before opening to minimize condensation.
Important Notes
Practical considerations for consistency and safety.
- Use new sterile insulin syringes for each injection; dispose in a sharps container[9].
- Rotate injection sites systematically (abdomen, thighs, upper arms) to reduce lipohypertrophy and local irritation[10].
- Inject slowly and wait a few seconds before withdrawing the needle.
- GHRP-6 may increase appetite in some users due to its ghrelin-mimicking properties[11].
- Document daily doses, timing, and sites to maintain consistency throughout the cycle.
How This Works
GHRP-6 functions as a synthetic ghrelin mimetic by binding to the growth hormone secretagogue receptor (GHS-R1a) in the pituitary gland and hypothalamus[1][12]. This activation triggers acute, pulsatile growth hormone release from somatotroph cells while simultaneously reducing somatostatin’s inhibitory brake on GH secretion[3]. Unlike continuous GH administration, GHRP-6 maintains physiological feedback controls—as GH and IGF-1 levels rise, endogenous somatostatin increases to prevent excessive elevation, keeping GH pulses within normal physiologic ranges[5].
Beyond its endocrine effects, GHRP-6 exhibits cytoprotective properties through interactions with the CD36 receptor on immune and muscle cells[2][13]. This secondary pathway activates cell-survival signaling cascades (such as PI3K/Akt) that help protect tissues from oxidative stress and inflammation, explaining many of GHRP-6’s observed tissue-protective benefits in preclinical models including cardioprotection, neuroprotection, and anti-fibrotic effects[2][14].
Potential Benefits & Side Effects
Observations from clinical and preclinical literature.
Potential Benefits:
- Muscle Growth & Fat Loss: Elevated GH and IGF-1 levels support increased lean body mass and reduced fat mass over time[7][15].
- Enhanced Recovery: Improved sleep architecture (increased slow-wave sleep) and faster tissue repair[16].
- Appetite Stimulation: May increase hunger due to ghrelin receptor activation, potentially beneficial for weight gain goals[11].
- Tissue Protection: Preclinical studies show cytoprotective effects including reduced scar formation, cardioprotection, and neuroprotection[2][14][17].
- Joint & Connective Tissue Health: May support collagen synthesis and connective tissue repair through elevated IGF-1[7].
Potential Side Effects:
- Transient mild increases in cortisol and ACTH (typically not clinically significant)[18].
- Increased appetite and potential water retention.
- Occasional injection-site reactions (redness, itching, mild swelling).
- Possible transient dizziness or flushed feeling immediately after injection due to rapid GH spike.
- Generally well-tolerated in human trials at research doses with no major safety concerns reported[19].
Lifestyle Factors
Complementary strategies for optimizing outcomes.
- Nutrition: Maintain adequate protein intake (1.6–2.2 g/kg body weight) to support anabolic processes. Time carbohydrate and fat intake away from injections to maximize GH release[6].
- Training: Combine resistance training with moderate cardiovascular activity to leverage elevated GH/IGF-1 for muscle growth and fat oxidation.
- Sleep: Prioritize 7–9 hours of quality sleep; GHRP-6’s effects on sleep architecture may enhance recovery[16].
- Hydration: Maintain proper hydration to support metabolic processes and minimize water retention.
- Stress Management: Chronic stress can interfere with GH secretion; incorporate stress-reduction techniques.
Injection Technique
General subcutaneous guidance from clinical best-practice resources[20][21].
- Preparation: Clean the vial stopper and injection site with alcohol swabs; allow to dry completely.
- Drawing the dose: Use a new sterile insulin syringe; draw the calculated units and check for air bubbles.
- Site selection: Rotate between abdomen (2+ inches from navel), outer thighs, and upper arms. Avoid repeatedly injecting the same spot[10][22].
- Technique: Pinch a skinfold; insert needle at 45–90° angle into subcutaneous tissue (angle depends on needle length and body fat)[20][21].
- Administration: Do not aspirate for subcutaneous injections. Inject slowly and steadily; hold for a few seconds before withdrawing[20].
- Disposal: Immediately dispose of used syringes and needles in a puncture-proof sharps container. Never reuse needles[9].
Recommended Source
We recommend Pure Lab Peptides for high-purity GHRP-6 (2 mg).
Why Pure Lab Peptides?
- High-purity, third-party-tested peptides with batch Certificates of Analysis (COAs).
- Consistent quality control and ISO-aligned handling procedures.
- Reliable fulfillment with proper cold-chain maintenance.
- Transparent documentation and responsive customer support.
Important Note
This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment. GHRP-6 is intended for research purposes only. Consult qualified healthcare professionals before beginning any peptide protocol. Individual responses may vary, and proper medical supervision is essential for safety and efficacy monitoring.
References
-
European Journal of Endocrinology (1997)
— Growth hormone-releasing peptides: comprehensive review of GHRP mechanisms and effects -
Clinical Medicine Insights: Cardiology (2017)
— Synthetic GHRPs: historical appraisal of cytoprotective effects and mechanisms -
Wikipedia
— GHRP-6: structure, pharmacology, and mechanism of action overview -
European Journal of Pharmaceutical Sciences (2013)
— Pharmacokinetic study of GHRP-6 in healthy male volunteers -
European Journal of Endocrinology (1997)
— Pulsatile GH secretion and feedback mechanisms with GHRPs -
Clinical Medicine Insights: Cardiology (2017)
— Effect of nutrient intake on GHRP-induced GH release -
Sexual Medicine Reviews (2018)
— Safety and efficacy of growth hormone secretagogues -
Assay Genie Product Datasheet (2021)
— GHRP-6 recombinant protein: storage and handling recommendations -
CDC (2024)
— Preventing unsafe injection practices: basic injection safety guidelines -
CDC (2024)
— 4 ways to take insulin: injection sites and rotation strategies -
Clinical Medicine Insights: Cardiology (2017)
— Ghrelin receptor activation and orexigenic effects of GHRP-6 -
Wikipedia
— GHRP-6 mechanism: ghrelin receptor binding and GH secretagogue activity -
Clinical Medicine Insights: Cardiology (2017)
— CD36 receptor activation and cell-survival signaling pathways -
International Wound Journal (2018)
— GHRP-6 prevents cutaneous hypertrophic scarring: proteome study -
Sexual Medicine Reviews (2018)
— GH secretagogues: effects on lean body mass and fat reduction -
Clinical Medicine Insights: Cardiology (2017)
— GHRP effects on sleep architecture and slow-wave sleep -
Clinical Medicine Insights: Cardiology (2017)
— Cardioprotective and neuroprotective effects of GHRPs in preclinical models -
Clinical Medicine Insights: Cardiology (2017)
— Transient ACTH and cortisol elevation with GHRP-6 administration -
Sexual Medicine Reviews (2018)
— Safety profile of growth hormone secretagogues in human trials -
CDC (2024)
— Vaccine administration: subcutaneous injection technique (fact sheet) -
CDC (2024)
— Vaccine administration: during vaccination best practices -
Clinical Diabetes (2019)
— The injection technique factor: what you don’t know or teach can make a difference -
Pure Lab Peptides
— GHRP-6 (2 mg) product page: quality assurance and batch documentation