GHK-Cu (50 mg Vial) Dosage Protocol
Quickstart Highlights
GHK-Cu (50 mg) dosage protocol focuses on supporting skin health, tissue regeneration, and overall wellness through once-daily subcutaneous injections.
- Typical daily dose ranges from 1 mg to 2 mg
- Once-daily injection schedule for consistency
- Recommended 3 mL reconstitution volume for flexible dosing
- Use smaller insulin syringes if measurement under 10 units is required
- Store lyophilized vials in freezer; reconstituted solution in refrigerator

Dosing & Reconstitution Guide
Educational guide for reconstitution and dosing protocol
Standard / Gradual Approach (3 mL = ~16.7 mg/mL)
Week | Daily Dosage (mg) | Units (per injection) |
---|---|---|
Weeks 1–4 | 1 mg once daily | ~6 units (0.06 mL) |
Weeks 5–8 | 2 mg once daily | ~12 units (0.12 mL) |
Reconstitute one 50 mg vial with 3 mL of bacteriostatic water (do not exceed 3 mL). This creates a ~16.7 mg/mL solution.
Note: A 1 mg dose is about 6 units, which is under 10 units. For greater precision, consider using smaller (50-unit or 30-unit) insulin syringes.
- Draw 3.0 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly along the vial wall to reduce foaming.
- Gently swirl the vial—avoid vigorous shaking.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
Protocol Overview
A concise summary of this once-daily regimen.
- Goal: Support skin health, tissue repair, and overall recovery
- Schedule: Daily subcutaneous injections for 8–12 weeks (or longer)
- Dose Range: Typically 1–2 mg daily
- Reconstitution: Use up to ~3 mL for a ~16.7 mg/mL concentration
- Storage: Keep lyophilized vials frozen; refrigerate after reconstitution
Dosing Protocol
Suggested once-daily injection approach to support collagen synthesis and tissue regeneration.
- Daily Dose: Start at 1 mg; may increase to 2 mg
- Frequency: Inject once per day subcutaneously
- Cycle Length: 8–12 weeks (extend based on research goals)
- Maximum Dose: Typically 2 mg/day per commonly referenced usage
- Timing: Any time of day; consistency is recommended
Storage Instructions
Proper storage ensures peptide integrity.
- Lyophilized: Freeze at −20°C until mixing
- Reconstituted: Refrigerate at 2–8°C
- Use within 30 days of reconstitution
- Avoid repeated freeze-thaw cycles
Supplies Needed
Ensure you have these on hand for an 8–16 week cycle.
- Peptide Vials:
• 8 wks ≈ 2 vials (1 mg first 4 wks, 2 mg next 4 wks ~ 84 mg total)
• 12 wks ≈ 3 vials
• 16 wks ≈ 4 vials
(Adjust if dosing above 2 mg/day or extending the cycle) - Insulin Syringes:
• 8 wks ≈ 56 syringes (1 per day)
• 12 wks ≈ 84+ syringes
Consider smaller syringes (50-unit/30-unit) for better precision - Bacteriostatic Water: 1× 30ml
- Alcohol Swabs: 1 box
Important Notes
Practical tips to enhance safety and efficacy.
- Always use new, sterile insulin syringes & rotate injection sites.
- Maintain consistency; daily injections yield the best results.
- Observe for adverse reactions; consult a professional if concerns arise.
- Track progress throughout the protocol, adjusting dosage as needed.
How This Works
GHK-Cu is a naturally occurring copper peptide known for its role in collagen synthesis and tissue repair.
- Skin & Tissue Support: Aids in the production of collagen and elastin
- Anti-Inflammatory: May help reduce inflammatory markers
- Promotes cell turnover, wound healing, and overall rejuvenation
Potential Benefits & Side Effects
Most users observe improvements in skin, hair, and general recovery, though results can vary.
- Supports collagen production and tissue regeneration
- May enhance wound healing and reduce inflammation
- Possible mild side effects: localized irritation, rare headaches
- Discontinue if you experience unusual reactions and consult a professional
Lifestyle Factors
Complementary strategies for maximum benefit.
- Adopt a balanced, nutrient-rich diet for skin and tissue health
- Incorporate regular exercise and prioritize proper recovery
- Manage stress levels and aim for adequate sleep
Injection Technique
Simple guidelines for safe daily injections.
- Clean vial rubber stopper & injection site with alcohol swabs
- Insert needle at a 45–90° angle into subcutaneous tissue
- Inject slowly & rotate sites (abdomen, thigh, etc.)
Recommended Source
We recommend Pure Lab Peptides for high-purity GHK-Cu (50 mg).
Why Pure Lab Peptides?
- Verifies ≥99% purity through independent lab testing
- Trusted by researchers seeking reliable results
- Follows rigorous manufacturing standards for consistent quality
Important Note
This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.
References
-
Google Patents
– WO2019167070A1 on GHK-Cu applications -
PubMed Central
– GHK-Cu clinical findings -
PubMed Central
– Collagen synthesis and wound healing -
Wiley Online Library
– GHK-Cu research in dermatology -
PubMed Central
– Additional GHK-Cu regenerative study -
Tocris Bioscience
– Calculator for solution preparation