GHK-Cu (100 mg Vial) Dosage Protocol

Quickstart Highlights

GHK-Cu (100 mg) dosage protocol focuses on supporting skin health, tissue regeneration, and overall wellness through once-daily subcutaneous injections.

  • Typical daily dose ranges from 1 mg to 2 mg
  • Once-daily injection schedule for consistency
  • Recommended 3 mL reconstitution volume for flexible dosing
  • Use smaller insulin syringes if measurement under 10 units is required
  • Store lyophilized vials in freezer; reconstituted solution in refrigerator
GHK-Cu Vial

Dosing & Reconstitution Guide

Educational guide for reconstitution and dosing protocol

Standard / Gradual Approach (3 mL = ~33.3 mg/mL)

Week Daily Dosage (mg) Units (per injection)
Weeks 1–4 1 mg once daily ~3 units (0.03 mL)
Weeks 5–8 2 mg once daily ~6 units (0.06 mL)

Reconstitute one 100 mg vial with 3 mL of bacteriostatic water (do not exceed 3 mL). This creates a ~33.3 mg/mL solution.
Note: Each 1 mg dose is approximately 3 units on a 100-unit syringe. Because this is under 10 units, using a smaller (50-unit or 30-unit) insulin syringe is recommended for better accuracy.

  1. Draw 3.0 mL of bacteriostatic water into a sterile syringe.
  2. Inject the water slowly along the vial wall to reduce foaming.
  3. Gently swirl the vial—avoid vigorous shaking.
  4. Label the vial with the reconstitution date and store at 2–8°C.
  5. Use within 30 days; avoid freeze-thaw cycles once mixed.

Note: This guide is for educational purposes only. Always consult with a qualified healthcare professional.

Protocol Overview

A concise summary of this once-daily regimen.

  • Goal: Support skin health, tissue repair, and overall recovery
  • Schedule: Daily subcutaneous injections for 8–12 weeks (or longer)
  • Dose Range: Typically 1–2 mg daily
  • Reconstitution: Use up to ~3 mL for a ~33.3 mg/mL concentration
  • Storage: Keep lyophilized vials frozen; refrigerate after reconstitution

Dosing Protocol

Suggested once-daily injection approach to support collagen synthesis and tissue regeneration.

  • Daily Dose: Start at 1 mg; may increase to 2 mg
  • Frequency: Inject once per day subcutaneously
  • Cycle Length: 8–12 weeks (extend based on research goals)
  • Maximum Dose: Typically 2 mg/day per commonly referenced usage
  • Timing: Any time of day; consistency is recommended

Storage Instructions

Proper storage ensures peptide integrity.

  • Lyophilized: Freeze at −20°C until mixing
  • Reconstituted: Refrigerate at 2–8°C
  • Use within 30 days of reconstitution
  • Avoid repeated freeze-thaw cycles

Supplies Needed

Ensure you have these on hand for an 8–16 week cycle.

  • Peptide Vials:
    • 8 wks ≈ 1 vial (if following 1 mg → 2 mg progression)
    • 12 wks ≈ 2 vials (depending on higher daily dosage)
    (Adjust if dosing above 2 mg/day or extending the cycle)
  • Insulin Syringes:
    • 8 wks ≈ 56 syringes (1 per day)
    • 12 wks ≈ 84+ syringes
    Consider smaller syringes (50-unit/30-unit) for better precision
  • Bacteriostatic Water: 1× 30ml
  • Alcohol Swabs: 1 box

Important Notes

Practical tips to enhance safety and efficacy.

  • Always use new, sterile insulin syringes & rotate injection sites.
  • Maintain consistency; daily injections yield the best results.
  • Observe for adverse reactions; consult a professional if concerns arise.
  • Track progress throughout the protocol, adjusting dosage as needed.

How This Works

GHK-Cu is a naturally occurring copper peptide known for its role in collagen synthesis and tissue repair.

  • Skin & Tissue Support: Aids in the production of collagen and elastin
  • Anti-Inflammatory: May help reduce inflammatory markers
  • Promotes cell turnover, wound healing, and overall rejuvenation

Potential Benefits & Side Effects

Most users observe improvements in skin, hair, and general recovery, though results can vary.

  • Supports collagen production and tissue regeneration
  • May enhance wound healing and reduce inflammation
  • Possible mild side effects: localized irritation, rare headaches
  • Discontinue if you experience unusual reactions and consult a professional

Lifestyle Factors

Complementary strategies for maximum benefit.

  • Adopt a balanced, nutrient-rich diet for skin and tissue health
  • Incorporate regular exercise and prioritize proper recovery
  • Manage stress levels and aim for adequate sleep

Injection Technique

Simple guidelines for safe daily injections.

  • Clean vial rubber stopper & injection site with alcohol swabs
  • Insert needle at a 45–90° angle into subcutaneous tissue
  • Inject slowly & rotate sites (abdomen, thigh, etc.)

Important Note

This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.

References

  • Google Patents – WO2019167070A1 on GHK-Cu applications
  • PubMed Central – GHK-Cu clinical findings
  • PubMed Central – Collagen synthesis and wound healing
  • Wiley Online Library – GHK-Cu research in dermatology
  • PubMed Central – Additional GHK-Cu regenerative study
  • Tocris Bioscience – Calculator for solution preparation

 

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