Epithalon (10mg Vial) Dosage Protocol
Quickstart Highlights
Epithalon 10mg dosage protocol is commonly referenced in anti-aging and longevity research, focusing on potential telomerase activation and pineal gland support.
- Well-known protocols include 10-day intensive or extended multi-injection schedules
- Typical total course range: 50–100 mg per cycle (multiple vials needed)
- Subcutaneous or intramuscular injections are commonly used
- Reconstitute carefully to ensure accurate dosing
- Store lyophilized vials in freezer; refrigerate after mixing

Dosing & Reconstitution Guide
Educational guide for reconstitution and dosing protocol
Standard / Gradual Approach (1 mL = 10 mg/mL)
Day | Dosage (mg) | Units (per injection) |
---|---|---|
Day 1 | 10 mg | 100 units |
Day 5 | 10 mg | 100 units |
Day 9 | 10 mg | 100 units |
Day 13 | 10 mg | 100 units |
Day 17 | 10 mg | 100 units |
This extended approach (sometimes called the “Ukrainian protocol”) spreads out 5 total injections of 10 mg each. Reconstitute the entire 10 mg vial with 1 mL to achieve a 10 mg/mL solution, then inject the full contents (100 units) on the specified days.
- Draw 1.0 mL of bacteriostatic water into a sterile syringe.
- Slowly inject the water along the vial wall to reduce foaming.
- Gently swirl until fully dissolved—avoid vigorous shaking.
- Label the vial with date and keep refrigerated at 2–8°C.
- Use each vial on the designated day; discard if contamination occurs.
Advanced / Aggressive Approach (1 mL = 10 mg/mL)
Day Range | Daily Dosage (mg) | Units (per injection) |
---|---|---|
Days 1–10 | 10 mg per day | 100 units |
Also known as the “Russian protocol,” this approach calls for 10 mg daily for 10 consecutive days (total 100 mg). Reconstitute each 10 mg vial with 1 mL to form a 10 mg/mL solution. You will need a new vial daily.
- Draw 1.0 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly to prevent peptide denaturation.
- Gently swirl—do not shake vigorously.
- Inject subcutaneously (100 units) once per day for 10 days.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
Protocol Overview
A concise summary of the primary Epithalon research protocols.
- Goal: Investigate potential anti-aging effects through telomerase activation
- Schedules: 10-day daily “intensive” or ~17-day “extended” regimen
- Total Dosage: Varies from 50 mg to 100 mg per cycle
- Reconstitution: 1 mL is often used for straightforward 10 mg/mL dosing
- Storage: Freeze vials until mixing; refrigerate after reconstitution
Dosing Protocol
Epithalon may be administered subcutaneously or intramuscularly. The total number of injections depends on the chosen protocol (10 or 5 total injections).
- Frequency (Advanced): 10 mg daily for 10 days
- Frequency (Standard): 10 mg once every 4 days (total 5 injections)
- Cycle Length: Typically 10–17 days; can repeat every 4–6 months
- Injection Method: Subcutaneous in the abdomen area or IM in the deltoid/glute
- Timing: Flexibility in injection time; consistency is encouraged
Storage Instructions
Proper handling and storage help maintain peptide integrity.
- Lyophilized: Keep frozen (−20°C) until use
- Reconstituted: Refrigerate at 2–8°C; use within recommended time frame
- Protect from light and discard if discoloration or particles appear
- Avoid multiple freeze-thaw cycles
Supplies Needed
Estimate your supply requirements based on the selected protocol.
- Peptide Vials:
• 10-Day Intensive ≈ 10 vials (if using 10 mg daily)
• 17-Day Extended ≈ 5 vials (if administering 10 mg on Days 1, 5, 9, 13, 17) - Insulin Syringes: 1 syringe per injection day (plus extras for safety)
- Bacteriostatic Water: 1× 30ml vial (adequate for multiple reconstitutions)
- Alcohol Swabs: 1 box (for vial tops and injection site prep)
Important Notes
Practical tips to enhance safety and consistency.
- Use sterile insulin syringes; dispose after each injection.
- Alternate injection sites to minimize local irritation.
- Monitor how you respond over the full course; adjust if recommended by a professional.
- Document doses and injection dates, especially for multi-day protocols.
How This Works
Epithalon (Epitalon) is a synthetic tetrapeptide believed to support healthy aging through multiple mechanisms.
- Telomerase Activation: May help preserve or extend telomere length
- Pineal Gland Support: Influences melatonin secretion and circadian rhythm
- Potential effects on antioxidant defense and immune modulation
Potential Benefits & Side Effects
Observational studies suggest notable anti-aging benefits, though individual results can vary.
- May support healthier sleep cycles and circadian rhythm
- Reported improvement in overall well-being
- Possible mild side effects: local injection soreness, transient headache, or dizziness
- Discontinue use and consult a professional if adverse reactions occur
Lifestyle Factors
Supplement peptide research with healthy daily habits.
- Maintain a balanced diet rich in nutrients
- Incorporate regular physical activity and sufficient rest
- Manage stress levels through mindfulness or relaxation techniques
Injection Technique
Simple guidelines for safe daily injections.
- Clean vial rubber stopper & injection site with alcohol swabs
- Insert needle at a 45–90° angle into subcutaneous tissue
- Inject slowly & rotate sites (abdomen, thigh, etc.)
Recommended Source
We recommend Pure Lab Peptides for high-purity BPC-157 + TB-500 blend.
Why Pure Lab Peptides?
- Verifies ≥99% purity through independent lab testing
- Trusted by researchers seeking reliable results
- Follows rigorous manufacturing standards for consistent quality
Important Note
This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.
References
-
PubMed
– Study on peptide aging markers -
Alzheimer’s Drug Discovery Foundation
– Epithalamin and Epithalon overview -
University of Washington
– Resource on administering micrograms -
PubMed
– Clinical insights on peptide therapy -
PubMed
– Investigation of peptide-based interventions -
PubMed Central
– Peer-reviewed article on epigenetic aging -
PubMed
– Research on telomerase modulation -
Science.gov
– Database entry on RGD peptides -
PubMed
– Additional findings on epitalon efficacy -
Duke Scholars
– Academic record on peptide research -
Frontiers in Aging
– Peer-reviewed discussion on aging -
PubMed
– Investigative article on longevity peptides