DSIP (10mg Vial) Dosage Protocol
Quickstart Highlights
DSIP (Delta Sleep‑Inducing Peptide) has been investigated for its effects on sleep architecture in controlled human studies. Published clinical trials most commonly used a weight‑based, single daily dose of ~25 nmol/kg (≈21 µg/kg), typically by slow intravenous administration over 1–4 days. The guide below converts that literature dose into practical volumes from a 10 mg (3 mL) vial.
- Most‑studied human dose: ~25 nmol/kg (≈21 µg/kg) per injection
- Frequency: 1× daily; courses of 1–4 consecutive days were commonly explored
- Reconstitution: Use the full 3 mL (max vial capacity) → ~3.33 mg/mL for easy unit counting
- Units math: At 3 mL, each 100‑unit insulin syringe unit ≈ 33 µg DSIP
- Storage: Lyophilized vials frozen; reconstituted solution refrigerated (details below)
Dosing & Reconstitution Guide
Educational guide translating literature dosing into practical volumes
Standard / Literature‑Derived Approach (3 mL = 3.33 mg/mL)
Target per‑injection dose: ~25 nmol/kg (≈21 µg/kg). Administer once daily for 1–4 days based on study designs. The table below converts body weight to approximate per‑injection dose and syringe units when the 10 mg vial is reconstituted to 3.0 mL.
| Body Weight | Dosage (per injection) | Units (per injection) (mL) |
|---|---|---|
| 50 kg | ~1,061 µg (1.061 mg) | 32 units (0.32 mL) |
| 60 kg | ~1,273 µg (1.273 mg) | 38 units (0.38 mL) |
| 70 kg | ~1,485 µg (1.485 mg) | 45 units (0.45 mL) |
| 80 kg | ~1,698 µg (1.698 mg) | 51 units (0.51 mL) |
| 90 kg | ~1,910 µg (1.910 mg) | 57 units (0.57 mL) |
| 100 kg | ~2,122 µg (2.122 mg) | 64 units (0.64 mL) |
Notes: Values assume reconstitution to 3.0 mL (10 mg ÷ 3 mL ≈ 3.33 mg/mL). Each 1 unit (on a 100‑unit insulin syringe) = 0.01 mL ≈ 33 µg DSIP. If using a different volume, recalculate proportionally. Literature most often reports slow intravenous administration; routes can vary by study. When injection volumes are small (<10 units), consider 30‑unit or 50‑unit syringes for finer measurement—however, at 3 mL dilution, units per injection are generally ≥10 across common body weights.
Reconstitution (3.0 mL total):
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall to minimize foaming; do not shake.
- Gently swirl until dissolved. Protect from light.
- Refrigerate the reconstituted solution at 2–8 °C. Avoid repeated freeze–thaw.
Supplies Needed
Plan based on the number of injections (1× daily for 1–4 days in many studies). One 10 mg vial reconstituted to 3 mL typically provides ~6 doses at ~1.5 mg each (≈70 kg reference). Adjust for body weight using the table above.
- Peptide Vials (10 mg):
• 1–4 doses ≈ 1 vial
• 5–8 doses ≈ 1–2 vials (≥70 kg likely 2)
• 9–12 doses ≈ 2 vials - Insulin Syringes: one per injection (plus spares)
- Bacteriostatic Water: 1× 30 mL
- Alcohol Swabs: 1 box
Protocol Overview
Concise summary of literature‑based dosing used in clinical investigations.
- Primary objective in studies: Modulation of sleep architecture (e.g., total sleep time, awakenings, sleep latency)
- Schedule: 1× daily dosing for 1–4 days was commonly studied
- Dose Basis: ~25 nmol/kg (≈21 µg/kg) per injection
- Reconstitution: Use 3.0 mL for a practical 3.33 mg/mL concentration
- Storage: Lyophilized at ≤−20 °C; reconstituted at 2–8 °C; avoid freeze–thaw
Dosing Protocol
How to interpret the literature dose for practical measurement.
- Per‑injection dose: ~21 µg/kg (i.e., 0.021 mg/kg)
- Frequency: Once daily (morning or pre‑sleep timing explored)
- Course length: 1–4 days reported; longer use has limited controlled data
- Unit conversion: With 3 mL dilution, 1 unit ≈ 33 µg; multiply dose (µg) ÷ 33 to get units
- Example (70 kg): ~1.485 mg → ~0.45 mL → ~45 units
Storage Instructions
General peptide stability practices from laboratory guidance.
- Lyophilized: Store at ≤−20 °C in a dry, dark environment; minimize moisture exposure
- Reconstituted: Refrigerate at 2–8 °C; aliquot if needed and avoid repeated freeze–thaw cycles
- Light & air: Protect from light; limit air exposure for solutions
Important Notes
Practical considerations when translating published dosing to measured volumes.
- Use new, sterile syringes and rotate injection sites when applicable to your protocol.
- Consistency in timing (daily at the same time) helps with standardized observations in study logs.
- Monitor for unexpected reactions and discontinue if adverse effects occur; discuss concerns with a qualified professional.
- Document weight, dose (µg/kg), units, time of day, and any observed sleep metrics to compare with published outcomes.
How This Works
DSIP is a nonapeptide (~849 Da) reported to cross the blood–brain and blood–CSF barriers in animal models and has been explored in human sleep studies.
- Neurophysiology: Modulation of EEG patterns linked to slow‑wave (delta) sleep has been observed in several studies and reviews.
- Hormonal/Stress Axes: Investigations have explored effects on ACTH/cortisol dynamics and interactions with other neuroendocrine pathways.
- Routes Studied: Intravenous (slow infusion), intranasal, and (in animals) subcutaneous; human literature most often used IV.
Potential Benefits & Side Effects
Clinical findings vary across trials; some reported improvements in sleep parameters (e.g., total sleep time, awakenings), while others found limited clinical significance. Overall tolerability was generally described as good in short‑term studies.
- Reported outcomes include changes in total sleep time, sleep latency, awakenings, and sleep efficiency
- Open‑label and small controlled studies suggest possible short‑term benefits; effects may be modest and inconsistent
- Short‑term studies reported few adverse effects; rigorous long‑term safety data are limited
Lifestyle Factors
When evaluating sleep‑related outcomes, consistent routines improve interpretability.
- Maintain regular sleep/wake times and a dark, quiet sleep environment
- Limit stimulants late in the day and manage evening light exposure
- Support sleep quality with balanced nutrition, stress management, and regular physical activity (not close to bedtime)
Injection Technique
General subcutaneous technique guidance (if applicable to your protocol); some DSIP studies used intravenous administration.
- Clean the vial stopper and skin with an alcohol swab
- Use fine‑gauge insulin syringes; inject slowly and rotate sites (abdomen, thigh)
- Dispose of sharps safely after each injection
Recommended Source
We recommend Pure Lab Peptides for DSIP (10 mg) with rigorous quality controls and documentation.
Why Pure Lab Peptides?
- Independent purity verification and batch documentation
- Transparent quality processes aligned with high laboratory standards
- Consistent formulation enabling precise measurement
Important Note
This guide is for educational purposes only and is not medical advice. Always consult a qualified healthcare professional with any questions about peptides.
References
-
Int J Clin Pharmacol Ther Toxicol
– Acute and delayed effects of DSIP on human sleep (25 nmol/kg IV) -
European Neurology
– DSIP in insomnia (summary of clinical investigations) -
European Neurology
– A clinical trial with DSIP (open study, repeated injections) -
Int J Clin Pharmacol Ther
– DSIP efficacy in chronic insomniac patients (double‑blind IV, 25 nmol/kg) -
J Clin Psychopharmacol
– Intranasal DSIP and P300 in humans -
Eur J Anaesthesiol
– Review of DSIP (sleep‑promoting properties and dosing observations) -
Brain Research
– Evidence that radiolabeled DSIP crosses the blood–brain barrier (rats) -
Pharmacol Biochem Behav
– DSIP crossing the blood–brain barrier in dogs; protein binding correlations -
Pharmacol Biochem Behav
– Passage of DSIP across the blood‑CSF barrier -
Physiology & Behavior
– Subcutaneous DSIP increases deep slow‑wave sleep in cats (animal model) -
Neuroscience Letters
– IV DSIP increases sleep in free‑moving rats (dose range 30–80 nmol/kg) -
Psychoneuroendocrinology
– Reduction of plasma ACTH after single DSIP dose in healthy men -
PubChem
– DSIP molecular weight and identifiers (C35H48N10O15; ~848.8 Da) -
NIBSC (UK)
– General peptide storage practices (lyophilized & solution) -
Clin Proteomics (PMC)
– Recommendations for peptide storage & handling for analytical work