TB-500 (5mg Vial) & BPC-157 (5mg Vial) Dosage Protocol
Quickstart Highlights
TB-500 & BPC-157 dosage protocol combines two research peptides often used together for synergistic tissue repair and recovery.
- TB-500 (5 mg/week standard) injected once or twice weekly
- BPC-157 ~500 mcg/day (can be split into 1–2 daily doses)
- Recommended 4–6 week cycle or longer, based on research needs
- Reconstitute each vial with bacteriostatic water up to 3 mL
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
Dosing & Reconstitution Guide
Educational guide for reconstitution and dosing protocol
TB-500 (5 mg Vial): Standard / Gradual Approach
Reconstitute with 2 mL of bacteriostatic water to achieve a concentration of 2.5 mg/mL. Each full vial = 5 mg total.
| Week | Weekly Total | Per Injection | Units (mL) | Frequency |
|---|---|---|---|---|
| Weeks 1–4 | 5 mg/week | 2.5 mg | ~100 units (1 mL) | 2× per week |
This approach splits the 5 mg weekly dose into two subcutaneous injections. At 2.5 mg per injection, you’ll draw about 1 mL (100 units) if reconstituted to 2 mL total.
- Draw 2.0 mL of bacteriostatic water into a sterile syringe.
- Inject slowly along the vial wall to minimize foam.
- Gently swirl the vial—avoid vigorous shaking.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
TB-500 (5 mg Vial): Advanced / Aggressive Approach
Reconstitute with 1 mL of bacteriostatic water for a 5 mg/mL solution.
| Week | Weekly Total | Per Injection | Units (mL) | Frequency |
|---|---|---|---|---|
| Weeks 1–4 | 10 mg/week | 5 mg | ~100 units (1 mL) | 2× per week |
An advanced protocol may push to 10 mg weekly by administering 5 mg twice per week. Mixing with just 1 mL of water yields ~5 mg per 1 mL (100 units). If 5 mg in a single injection feels too high, split it into smaller doses.
- Draw 1.0 mL of bacteriostatic water into a sterile syringe.
- Inject slowly along the vial wall.
- Gently swirl—avoid vigorous shaking.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
BPC-157 (5 mg Vial): Standard / Gradual Approach
Reconstitute with 2 mL of bacteriostatic water, resulting in 2.5 mg/mL. Ideal for daily or split-daily injections.
| Week | Daily Total (mcg) | Per Injection | Units (mL) | Frequency |
|---|---|---|---|---|
| Weeks 1–2 | 500 mcg/day | 250 mcg | ~10 units (0.1 mL) | 2× per day |
| Weeks 3–4 | 500 mcg/day | 500 mcg | ~20 units (0.2 mL) | 1× per day |
Gradually taper from 2 smaller doses to a single daily dose if desired. With 2 mL total volume, 1 mg is ~0.4 mL (40 units). Typical ~500 mcg/day usage remains easy to measure above 10 units.
- Draw 2.0 mL of bacteriostatic water into a sterile syringe.
- Inject along the vial wall slowly to reduce foaming.
- Gently swirl—avoid shaking vigorously.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
BPC-157 (5 mg Vial): Advanced / Aggressive Approach
Reconstitute with 1 mL of bacteriostatic water for a 5 mg/mL concentration.
| Week | Daily Total (mcg) | Per Injection | Units (mL) | Frequency |
|---|---|---|---|---|
| Weeks 1–2 | 750 mcg/day | ~375 mcg | ~7.5 units (0.075 mL) | 2× per day |
| Weeks 3–4 | 500 mcg/day | 500 mcg | ~10 units (0.1 mL) | 1× per day |
Smaller injection volumes can be harder to measure. If going below ~10 units, consider using a 50-unit or 30-unit syringe for better accuracy.
- Draw 1.0 mL of bacteriostatic water into a sterile syringe.
- Inject slowly; gently swirl until dissolved.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
Combining TB-500 & BPC-157 in a Stack
You can run both peptides simultaneously:
- TB-500: 5–10 mg weekly (1–2 injections each week)
- BPC-157: ~500 mcg/day, or an advanced protocol up to ~750 mcg/day
- Each peptide reconstituted in its own vial; do not mix in the same syringe
Users often report synergistic recovery effects with both peptides. Administering them on the same day is fine; just use separate syringes and injection sites if desired.
Protocol Overview
A concise weekly (TB-500) and daily (BPC-157) injection strategy.
- Goal: Tissue repair and accelerated recovery
- TB-500: 5–10 mg per week, administered 1–2× weekly
- BPC-157: 500–750 mcg per day, split into 1–2 doses
- Cycle Length: Generally 4–6 weeks, extend if needed
- Reconstitution: Aim for 2 mL total for simpler measuring
Dosing Protocol
Recommended approach for combining both peptides effectively.
- Injection Method: Subcutaneous, rotating sites
- TB-500 Schedule: Typically 2 injections per week (e.g., Monday & Thursday)
- BPC-157 Schedule: Daily or split daily for Weeks 1–4, optional taper afterward
- Timing: Separate syringes; no need for precise timing alignment
- Duration: 4+ weeks, re-assess based on progress
Storage Instructions
Proper handling preserves peptide quality.
- Lyophilized: Keep frozen until ready to mix
- Reconstituted: Store at 2–8°C; use within 30 days
- Protect from light; keep vials upright if possible
- Avoid repeated freeze-thaw cycles
Supplies Needed
Basic materials for a 4–6 week cycle.
-
Peptide Vials:
• TB-500 (5 mg Vial) – Product ID: 1040
• BPC-157 (5 mg Vial) – Product ID: 1035
(Adjust based on total duration) - Insulin Syringes: 1 per injection (30–100 unit size)
- Bacteriostatic Water: 1× 30 ml or as needed
- Alcohol Swabs: 1 box
Important Notes
Tips for safe, consistent usage.
- Use new, sterile insulin syringes every time; rotate injection sites.
- Maintain consistent protocol; peptides often show best results with regular use.
- Monitor for adverse reactions; consult a professional if needed.
- Do not mix TB-500 and BPC-157 in the same syringe.
How This Works
Both peptides are known for supporting tissue repair in various research settings.
- TB-500 (Thymosin Beta-4 Fragment): May aid in cell migration and angiogenesis.
- BPC-157: Investigated for potential benefits in gut healing and collagen synthesis.
- They work via distinct biological mechanisms but often stack well for synergistic effects.
Potential Benefits & Side Effects
Research suggests various therapeutic benefits, with some caution advised.
- May accelerate soft tissue repair and reduce inflammation
- Possible support for tendon, ligament, and muscle recovery
- Mild side effects reported: injection-site irritation, headache, dizziness
- Long-term safety remains under study
Lifestyle Factors
Simple habits can complement peptide usage.
- Adopt a balanced, protein-rich diet
- Engage in regular exercise; allow sufficient recovery
- Focus on stress management and adequate sleep
Injection Technique
Simple guidelines for safe daily injections.
- Clean vial rubber stopper & injection site with alcohol swabs
- Insert needle at a 45–90° angle into subcutaneous tissue
- Inject slowly & rotate sites (abdomen, thigh, etc.)
Recommended Source
We recommend Pure Lab Peptides for high-purity BPC-157 + TB-500 blend.
Why Pure Lab Peptides?
- Verifies ≥99% purity through independent lab testing
- Trusted by researchers seeking reliable results
- Follows rigorous manufacturing standards for consistent quality
References
-
Metabolic Pharmaceuticals Trial
– PatientPop PDF (Obesity / AOD-9604 Study) -
FDA Document
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FDA Document
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BioSpace
– Metabolic Pharmaceuticals AOD-9604 Trial Update -
PMC
– Article PMC3584306 (Peer-Reviewed Study) -
Endocrinology Journal
– AOD-9604 Research (GH Fragment) -
PubMed
– PMID: 11146367 (Hormone / Growth Factor Study) -
Annals of Clinical & Laboratory Science
– GH Fragments & Lipolysis Study -
PMC
– Article PMC3136748 (Peptide-Related Study) -
Endocrinology Journal (PDF)
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Journal of Endocrinology & Metabolism
– AOD-9604 Discussion (Article 157/194) -
FDA Document
– 2015-N-3534-0283 (Attachment 4) -
Elite Plastic Surgery AZ
– Peptide Therapy Insights -
Google Patents
– Patent WO2007033427A1 (GH Fragment Methods) -
Journal of Endocrinology & Metabolism
– AOD-9604 Clinical Usage (Article 213/278)