MOTS-C (10mg Vial) Dosage Protocol
Quickstart Highlights
MOTS-C 10mg dosage protocol is a mitochondrial-derived peptide that may support metabolic regulation, exercise performance, and healthy aging through periodic subcutaneous injections.
- Common research use: 5 mg every 5 days or 10 mg per injection (advanced)
- Flexible dosing schedule (e.g., once every 5 days or 3x weekly)
- Reconstitute for accurate insulin syringe measurements (up to 3 mL max)
- Store lyophilized in the freezer; reconstituted in the refrigerator
Dosing & Reconstitution Guide
Educational guide for reconstitution and dosing protocol
Standard / Gradual Approach (2 mL = 5 mg/mL)
| Injection # | Single Dose (mg) | Units (per injection) |
|---|---|---|
| Injection 1 (Day 1) | 5 mg | 100 units (1.0 mL) |
| Injection 2 (Day 6) | 5 mg | 100 units (1.0 mL) |
| Injection 3 (Day 11) | 5 mg | 100 units (1.0 mL) |
| Injection 4 (Day 16) | 5 mg | 100 units (1.0 mL) |
A common research protocol involves 5 mg injections every 5 days (4 total injections over ~20 days). Reconstitute with 2 mL to create a 5 mg/mL solution.
- Draw 2.0 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly along the vial wall to minimize foam.
- Gently swirl—avoid vigorous shaking.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
Advanced / Aggressive Approach (1 mL = 10 mg/mL)
| Week | Injections/Week | Single Dose (mg) | Units (per injection) |
|---|---|---|---|
| Weeks 1–4 | 3x weekly (e.g., M/W/F) | 10 mg | 100 units (1.0 mL) |
Recommended for higher total weekly dosing. Reconstitute with 1 mL to achieve a 10 mg/mL solution. Each injection is 1.0 mL (100 units).
Note: Multiple vials may be required if following this protocol over several weeks.
- Draw 1.0 mL of bacteriostatic water into a sterile syringe.
- Slowly inject the water to reduce foaming.
- Gently roll/swirl the vial—no vigorous shaking.
- Store the reconstituted solution at 2–8 °C (refrigerator), protected from light.
Protocol Overview
A concise summary of the MOTS-C protocol for metabolic and performance research.
- Goal: Investigate potential metabolic regulation and exercise performance benefits
- Schedule: Subcutaneous injections every 5 days or 3x per week, depending on protocol
- Dose Range: 5 mg to 10 mg per injection
- Reconstitution: Use 1–2 mL for practical measuring (up to 3 mL max)
- Storage: Keep lyophilized vials frozen; refrigerate after reconstitution
Dosing Protocol
Suggested approaches include a moderate “standard” schedule or an “advanced” higher dose regimen.
- Standard Frequency: Inject 5 mg once every 5 days (4 total injections over ~20 days)
- Advanced Frequency: Inject 10 mg up to 3 times weekly
- Cycle Length: Varies by research design (e.g., 4–8 weeks or longer)
- Timing: Subcutaneous administration; consistent injection timing suggested
Storage Instructions
Proper storage ensures peptide integrity.
- Lyophilized: Freeze until mixing (−20°C)
- Reconstituted: Refrigerate at 2–8°C
- Use within 30 days of reconstitution
- Avoid repeated freeze-thaw cycles
Supplies Needed
Ensure you have these on hand for a 4–8+ week study.
- Peptide Vials:
• 4 wks ≈ 1–3 vials (depending on dose/frequency)
• 8 wks ≈ 2–6 vials - Insulin Syringes:
• Quantity depends on injection frequency and cycle length - Bacteriostatic Water: 1× 30ml
- Alcohol Swabs: 1 box
Important Notes
Practical tips to enhance safety and efficacy.
- Always use new, sterile insulin syringes & rotate injection sites.
- Maintain consistency; follow the planned schedule for reliable results.
- Observe for adverse reactions; consult a professional if concerns arise.
- Track progress throughout the protocol, adjusting dosage as recommended.
How This Works
MOTS-C is a mitochondrial-derived peptide that may help regulate energy balance, glucose metabolism, and exercise performance.
- Metabolic Support: Influences insulin sensitivity and cellular energy pathways
- Physical Performance: May improve exercise capacity via mitochondrial function
- Possible anti-inflammatory effects noted in some research contexts
Potential Benefits & Side Effects
Most research indicates improvements in metabolism and endurance, though individual responses vary.
- May support healthier glucose regulation and energy expenditure
- Potential to enhance physical performance and recovery
- Possible mild side effects include fatigue, headache, or local injection-site irritation
- Rarely, oxidative stress or tissue fatigue could occur with overuse
Lifestyle Factors
Complementary strategies for maximum benefit.
- Adopt a balanced, nutrient-dense diet
- Engage in consistent exercise and prioritize adequate rest
- Manage stress levels and maintain good hydration
Injection Technique
Simple guidelines for safe daily injections.
- Clean vial rubber stopper & injection site with alcohol swabs
- Insert needle at a 45–90° angle into subcutaneous tissue
- Inject slowly & rotate sites (abdomen, thigh, etc.)
Recommended Source
We recommend Pure Lab Peptides for high-purity MOTS-C.
Why Pure Lab Peptides?
- Verifies ≥99% purity through independent lab testing
- Trusted by researchers seeking reliable results
- Follows rigorous manufacturing standards for consistent quality
Important Note
This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.
References
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PubMed Central
– Mitochondrial peptide regulation study -
EMBO Molecular Medicine
– MOTS-c and metabolic function -
Nature Communications
– Mitochondrial peptide benefits -
PubMed Central
– MOTS-c metabolic improvement research -
PubMed Central
– Studies on mitochondrial-derived peptides -
PubMed Central
– Recent findings on MOTS-c -
Alzheimer’s Drug Discovery
– Cognitive Vitality review of MOTS-c -
Frontiers in Physiology
– MOTS-c research article -
bioRxiv
– Preprint on MOTS-c function -
Diabetes & Metabolism Journal
– Examination of MOTS-c in diabetes -
PubMed Central
– Investigations on MOTS-c efficacy -
Frontiers in Endocrinology
– Endocrine impacts of MOTS-c -
Aging (Albany NY)
– MOTS-c and cellular aging -
USADA
– Anti-doping overview of MOTS-c -
Physiological Reports
– Peer-reviewed MOTS-c analysis