Cartalax (20mg Vial) Dosage Protocol

Cartalax (20 mg Vial) Dosage Protocol

Quickstart Highlights

Cartalax is a synthetic tripeptide bioregulator (Ala‑Glu‑Asp; sequence “AED”) developed by Prof. Vladimir Khavinson at the St. Petersburg Institute of Bioregulation and Gerontology[1]. Preclinical studies indicate it may modulate fibroblast proliferation markers (Ki‑67), reduce pro‑apoptotic signaling (p53, caspase‑3), and support extracellular matrix homeostasis[2][3]. Note: Published human posology for subcutaneous Cartalax is limited; this framework extrapolates from available preclinical and observational data for educational purposes only.

  • Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration.
  • Example daily range: 2,000–5,000 mcg once daily (gradual titration).
  • Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U‑100 insulin syringe.
  • Storage: Lyophilized: store at 2–8 °C (35.6–46.4 °F) or freeze at −20 °C (−4 °F) for long‑term; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
Cartalax 20 mg Vial

Dosing & Reconstitution Guide

Educational framework for reconstitution and daily dosing

Standard / Gradual Approach (3 mL = ~6.67 mg/mL)

Route & Frequency: Subcutaneous, once daily. Evidence note: Specific human RCT posology for SC Cartalax is limited; this schedule references preclinical fibroblast/chondrocyte studies and general SC technique guidance[2][8].

Week/Phase Daily Dose (mcg / mg) Units (per injection) (mL)
Weeks 1–2 2,000 mcg (2.0 mg) 30 units (0.30 mL)
Weeks 3–4 3,000 mcg (3.0 mg) 45 units (0.45 mL)
Weeks 5–8 4,000 mcg (4.0 mg) 60 units (0.60 mL)
Weeks 9–12 5,000 mcg (5.0 mg) 75 units (0.75 mL)

This schedule uses the largest practical dilution (3.0 mL) to keep per‑injection volumes within typical SC tolerability parameters (≤~1.0 mL per site)[9][10].

Reconstitution Steps

  1. Draw 3.0 mL bacteriostatic water with a sterile syringe.
  2. Insert needle through the stopper; let the diluent run slowly down the vial wall to avoid foaming.
  3. Gently swirl or roll until fully dissolved (do not shake).
  4. Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration (average ~3.5 mg/day).

  • Peptide Vials (Cartalax, 20 mg each):

    • 8 weeks ≈ 10 vials
    • 12 weeks ≈ 15 vials
    • 16 weeks ≈ 20 vials
  • Insulin Syringes (U‑100):

    • Per week: 7 syringes (1/day)
    • 8 weeks: 56 syringes
    • 12 weeks: 84 syringes
    • 16 weeks: 112 syringes
  • Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

    • 8 weeks (10 vials): 30 mL → 3 × 10 mL bottles
    • 12 weeks (15 vials): 45 mL → 5 × 10 mL bottles
    • 16 weeks (20 vials): 60 mL → 6 × 10 mL bottles
  • Alcohol Swabs: One for the vial stopper + one for the injection site each day.

    • Per week: 14 swabs (2/day)
    • 8 weeks: 112 swabs → recommend 2 × 100‑count boxes
    • 12 weeks: 168 swabs → recommend 2 × 100‑count boxes
    • 16 weeks: 224 swabs → recommend 3 × 100‑count boxes
Cartalax 20 mg Vial
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Bacteriostatic Water
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Protocol Overview

Concise summary of the once‑daily regimen.

  • Goal: Support connective‑tissue homeostasis and fibroblast function based on preclinical bioregulator research[2][3].
  • Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).
  • Dose Range: 2,000–5,000 mcg daily with gradual titration.
  • Reconstitution: 3.0 mL per 20 mg vial (~6.67 mg/mL) for accurate unit measurements.
  • Storage: Lyophilized: refrigerate or freeze; reconstituted: refrigerate; avoid repeated freeze–thaw.

Dosing Protocol

Suggested daily titration approach.

  • Start: 2,000 mcg daily; increase by ~1,000 mcg every 2 weeks as tolerated.
  • Target: 4,000–5,000 mcg daily by Weeks 5–12.
  • Frequency: Once per day (subcutaneous).
  • Cycle Length: 8–12 weeks; optional extension to 16 weeks.
  • Timing: Any consistent time; rotate injection sites.

Storage Instructions

Proper storage preserves peptide quality.

  • Lyophilized: Store at 2–8 °C (35.6–46.4 °F) or freeze at −20 °C (−4 °F) for long‑term stability; protect from light and moisture[11].
  • Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
  • Allow vials to reach room temperature before opening to reduce condensation uptake.

Important Notes

Practical considerations for consistency and safety.

  • Use new sterile insulin syringes; dispose in a sharps container.
  • Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
  • Inject slowly; wait a few seconds before withdrawing the needle.
  • Document daily dose and site rotation to maintain consistency.
  • Evidence caveat: Published human clinical trial data for subcutaneous Cartalax is limited; this protocol extrapolates from preclinical studies and standard peptide handling practices.

How This Works

Cartalax (Ala‑Glu‑Asp) is classified among the Khavinson bioregulatory peptides—ultrashort peptides that may interact with DNA and modulate gene expression at nanomolar concentrations[1][4]. The peptide sequence corresponds to a motif found in the alpha‑1 chain of type XI collagen, a structural protein important for cartilage integrity[5]. In preclinical fibroblast and chondrocyte culture models, Cartalax has been reported to upregulate Ki‑67 (a proliferation marker), increase SIRT‑1/SIRT‑6 expression, reduce p53 and caspase‑3 activity (pro‑apoptotic signals), and inhibit MMP‑9 synthesis (an enzyme linked to extracellular matrix degradation)[2][3][6].

Potential Benefits & Side Effects

Observations from preclinical literature.

  • May support fibroblast proliferation and reduce markers of cellular senescence in aged cell cultures[2][3].
  • Preclinical data suggest modulation of extracellular matrix homeostasis via MMP‑9 inhibition and collagen‑related gene expression[6].
  • General tolerability: Khavinson bioregulator peptides have been described as well tolerated in observational settings; occasional mild injection‑site reactions (redness, itch) may occur with subcutaneous administration[1].
  • Limitations: No large‑scale human RCTs; most data derive from in vitro or rodent models.

Lifestyle Factors

Complementary strategies for best outcomes.

  • Support joint and connective‑tissue health with adequate protein, vitamin C, and collagen precursors.
  • Combine low‑impact exercise and mobility work to reinforce musculoskeletal adaptations.
  • Prioritize sleep and stress management to support tissue repair and recovery.

Injection Technique

General subcutaneous guidance from clinical best‑practice resources[8][12].

  • Clean the vial stopper and skin with alcohol; allow to dry.
  • Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue[8].
  • Do not aspirate for subcutaneous injections; inject slowly and steadily[8].
  • Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy[12].

Important Note

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.

References


  • Neuroendocrinology Letters (2002)
    — Khavinson VK. Peptides and Ageing. Overview of bioregulatory peptide development and geroprotective mechanisms.
    View Source

  • Bulletin of Experimental Biology and Medicine (2016)
    — Lin’kova NS et al. Peptide Regulation of Skin Fibroblast Functions during Their Aging In Vitro. AED peptide effects on Ki‑67, CD98hc, caspase‑3, and MMP‑9.
    View Source

  • Bulletin of Experimental Biology and Medicine (2014)
    — Khavinson VK et al. Peptides regulate the expression of signaling molecules in kidney cell cultures during in vitro aging (p53, p16, SIRT‑6).
    View Source

  • Molecular Biology Reports (2020)
    — Ashapkin V, Khavinson V et al. Gene expression in human mesenchymal stem cell aging cultures: modulation by short peptides.
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  • International Journal of Molecular Sciences (2023)
    — Linkova N, Khavinson V et al. Peptide Regulation of Chondrogenic Stem Cell Differentiation.
    View Source

  • Advances in Gerontology (2020)
    — Khavinson VK, Linkova NS et al. Short peptides: regulation of skin function during aging (collagen, SIRT‑1/‑6, MMP regulation).
    View Source

  • PubChem
    — Compound summary for Cartalax (AED peptide; CID 87815447): molecular formula C₁₂H₁₉N₃O₈, MW 333.29.
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  • CDC
    — Vaccine administration: subcutaneous route (angle/site; no aspiration).
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  • Advances in Therapy (PubMed)
    — Subcutaneous injection factors and tolerability; practical volume considerations.
    View Source

  • StatPearls (NCBI Bookshelf)
    — Medication routes of administration; cautions for large single‑site SC volumes.
    View Source

  • Bachem
    — Handling and Storage Guidelines for Peptides (lyophilized and reconstituted stability).
    View Source

  • NCBI Bookshelf
    — Best practices for injection (asepsis, preparation, and administration).
    View Source

  • Pure Lab Peptides
    — Cartalax (20 mg) product page (quality and batch documentation).
    View Source