TB-500 (5mg Vial) & BPC-157 (5mg Vial) Dosage Protocol

Quickstart Highlights

TB-500 & BPC-157 dosage protocol combines two research peptides often used together for synergistic tissue repair and recovery.

  • TB-500 (5 mg/week standard) injected once or twice weekly
  • BPC-157 ~500 mcg/day (can be split into 1–2 daily doses)
  • Recommended 4–6 week cycle or longer, based on research needs
  • Reconstitute each vial with bacteriostatic water up to 3 mL
  • Keep reconstituted vials refrigerated (2–8°C) and use within 30 days
TB-500 Vial BPC-157 Vial

Dosing & Reconstitution Guide

Educational guide for reconstitution and dosing protocol

TB-500 (5 mg Vial): Standard / Gradual Approach

Reconstitute with 2 mL of bacteriostatic water to achieve a concentration of 2.5 mg/mL. Each full vial = 5 mg total.

Week Weekly Total Per Injection Units (mL) Frequency
Weeks 1–4 5 mg/week 2.5 mg ~100 units (1 mL) 2× per week

This approach splits the 5 mg weekly dose into two subcutaneous injections. At 2.5 mg per injection, you’ll draw about 1 mL (100 units) if reconstituted to 2 mL total.

  1. Draw 2.0 mL of bacteriostatic water into a sterile syringe.
  2. Inject slowly along the vial wall to minimize foam.
  3. Gently swirl the vial—avoid vigorous shaking.
  4. Label with date, then store at 2–8°C.
  5. Use within 30 days; avoid repeated freeze-thaw cycles.

TB-500 (5 mg Vial): Advanced / Aggressive Approach

Reconstitute with 1 mL of bacteriostatic water for a 5 mg/mL solution.

Week Weekly Total Per Injection Units (mL) Frequency
Weeks 1–4 10 mg/week 5 mg ~100 units (1 mL) 2× per week

An advanced protocol may push to 10 mg weekly by administering 5 mg twice per week. Mixing with just 1 mL of water yields ~5 mg per 1 mL (100 units). If 5 mg in a single injection feels too high, split it into smaller doses.

  1. Draw 1.0 mL of bacteriostatic water into a sterile syringe.
  2. Inject slowly along the vial wall.
  3. Gently swirl—avoid vigorous shaking.
  4. Refrigerate at 2–8°C and use within 30 days.

BPC-157 (5 mg Vial): Standard / Gradual Approach

Reconstitute with 2 mL of bacteriostatic water, resulting in 2.5 mg/mL. Ideal for daily or split-daily injections.

Week Daily Total (mcg) Per Injection Units (mL) Frequency
Weeks 1–2 500 mcg/day 250 mcg ~10 units (0.1 mL) 2× per day
Weeks 3–4 500 mcg/day 500 mcg ~20 units (0.2 mL) 1× per day

Gradually taper from 2 smaller doses to a single daily dose if desired. With 2 mL total volume, 1 mg is ~0.4 mL (40 units). Typical ~500 mcg/day usage remains easy to measure above 10 units.

  1. Draw 2.0 mL of bacteriostatic water into a sterile syringe.
  2. Inject along the vial wall slowly to reduce foaming.
  3. Gently swirl—avoid shaking vigorously.
  4. Keep refrigerated at 2–8°C; label the date.
  5. Dispose of reconstituted product after 30 days.

BPC-157 (5 mg Vial): Advanced / Aggressive Approach

Reconstitute with 1 mL of bacteriostatic water for a 5 mg/mL concentration.

Week Daily Total (mcg) Per Injection Units (mL) Frequency
Weeks 1–2 750 mcg/day ~375 mcg ~7.5 units (0.075 mL) 2× per day
Weeks 3–4 500 mcg/day 500 mcg ~10 units (0.1 mL) 1× per day

Smaller injection volumes can be harder to measure. If going below ~10 units, consider using a 50-unit or 30-unit syringe for better accuracy.

  1. Draw 1.0 mL of bacteriostatic water into a sterile syringe.
  2. Inject slowly; gently swirl until dissolved.
  3. Refrigerate at 2–8°C and discard after 30 days.

Combining TB-500 & BPC-157 in a Stack

You can run both peptides simultaneously:

  • TB-500: 5–10 mg weekly (1–2 injections each week)
  • BPC-157: ~500 mcg/day, or an advanced protocol up to ~750 mcg/day
  • Each peptide reconstituted in its own vial; do not mix in the same syringe

Users often report synergistic recovery effects with both peptides. Administering them on the same day is fine; just use separate syringes and injection sites if desired.

Note: This guide is for educational purposes only. Always consult a qualified healthcare professional.

Protocol Overview

A concise weekly (TB-500) and daily (BPC-157) injection strategy.

  • Goal: Tissue repair and accelerated recovery
  • TB-500: 5–10 mg per week, administered 1–2× weekly
  • BPC-157: 500–750 mcg per day, split into 1–2 doses
  • Cycle Length: Generally 4–6 weeks, extend if needed
  • Reconstitution: Aim for 2 mL total for simpler measuring

Dosing Protocol

Recommended approach for combining both peptides effectively.

  • Injection Method: Subcutaneous, rotating sites
  • TB-500 Schedule: Typically 2 injections per week (e.g., Monday & Thursday)
  • BPC-157 Schedule: Daily or split daily for Weeks 1–4, optional taper afterward
  • Timing: Separate syringes; no need for precise timing alignment
  • Duration: 4+ weeks, re-assess based on progress

Storage Instructions

Proper handling preserves peptide quality.

  • Lyophilized: Keep frozen until ready to mix
  • Reconstituted: Store at 2–8°C; use within 30 days
  • Protect from light; keep vials upright if possible
  • Avoid repeated freeze-thaw cycles

Supplies Needed

Basic materials for a 4–6 week cycle.

  • Peptide Vials:
    • TB-500 (5 mg Vial) – Product ID: 1040
    • BPC-157 (5 mg Vial) – Product ID: 1035
    (Adjust based on total duration)
  • Insulin Syringes: 1 per injection (30–100 unit size)
  • Bacteriostatic Water: 1× 30 ml or as needed
  • Alcohol Swabs: 1 box

Important Notes

Tips for safe, consistent usage.

  • Use new, sterile insulin syringes every time; rotate injection sites.
  • Maintain consistent protocol; peptides often show best results with regular use.
  • Monitor for adverse reactions; consult a professional if needed.
  • Do not mix TB-500 and BPC-157 in the same syringe.

How This Works

Both peptides are known for supporting tissue repair in various research settings.

  • TB-500 (Thymosin Beta-4 Fragment): May aid in cell migration and angiogenesis.
  • BPC-157: Investigated for potential benefits in gut healing and collagen synthesis.
  • They work via distinct biological mechanisms but often stack well for synergistic effects.

Potential Benefits & Side Effects

Research suggests various therapeutic benefits, with some caution advised.

  • May accelerate soft tissue repair and reduce inflammation
  • Possible support for tendon, ligament, and muscle recovery
  • Mild side effects reported: injection-site irritation, headache, dizziness
  • Long-term safety remains under study

Lifestyle Factors

Simple habits can complement peptide usage.

  • Adopt a balanced, protein-rich diet
  • Engage in regular exercise; allow sufficient recovery
  • Focus on stress management and adequate sleep

Injection Technique

Simple guidelines for safe daily injections.

  • Clean vial rubber stopper & injection site with alcohol swabs
  • Insert needle at a 45–90° angle into subcutaneous tissue
  • Inject slowly & rotate sites (abdomen, thigh, etc.)

Important Note

This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.

References

  • Metabolic Pharmaceuticals Trial – PatientPop PDF (Obesity / AOD-9604 Study)
  • FDA Document – Regulations Submission (Attachment 4)
  • FDA Document – Regulations Submission (Attachment 17)
  • BioSpace – Metabolic Pharmaceuticals AOD-9604 Trial Update
  • PMC – Article PMC3584306 (Peer-Reviewed Study)
  • Endocrinology Journal – AOD-9604 Research (GH Fragment)
  • PubMed – PMID: 11146367 (Hormone / Growth Factor Study)
  • Annals of Clinical & Laboratory Science – GH Fragments & Lipolysis Study
  • PMC – Article PMC3136748 (Peptide-Related Study)
  • Endocrinology Journal (PDF) – Advanced GH Fragment Research
  • Journal of Endocrinology & Metabolism – AOD-9604 Discussion (Article 157/194)
  • FDA Document – 2015-N-3534-0283 (Attachment 4)
  • Elite Plastic Surgery AZ – Peptide Therapy Insights
  • Google Patents – Patent WO2007033427A1 (GH Fragment Methods)
  • Journal of Endocrinology & Metabolism – AOD-9604 Clinical Usage (Article 213/278)

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